Based on columbia agar base enriched with 5% horse blood the medium is made selective by the inclusion of colistin and aztreonam to suppress the growth of the. Certain bacteria produce exotoxins called hemolysins, which act on the red blood cells to lyse, or break them down. For example, mannitol salt agar contains a high concentration of sodium chloride that inhibits the growth of most organisms but permits staphyl. Principles of macconkey agar, mannitol salt agar, blood. Usually sheep blood and sometimes horse or rabbit blood is preferred for the preparation blood agar media. Which agar would be most useful when examining a mixture of gramnegative bacteria. Blood agar plates and hemolysis protocols american society of. Blood agar bacterial growth medium science prof online. A suitable blood agar containing human blood especially for the use. Bordetella pertussis on bordetella selective medium charcoal horse blood agar with cefalexin. Such organisms do not grow well using ordinary growth medium. Blood agar base was used during investigations on irradiated escherichia coli and other bacteria 1,2.
It is treated and water and a number of other ingredients are added to sterilize the solution. Add 10% blood to columbia agar, mix well and supplement 5 mgl. Factors in agar composition antagonists of folate synthesis inhibitors paraaminobenzoic acid paba is a potent inhibitor of sulfonamides. Recommended with addition of sheep blood, gives improved haemolytic reactions of organisms. Baps are enriched, differential media used to isolate fastidious organisms and detect hemolytic activity.
Detection and identification of staphylococcus lugdunensis. Identification of product and supplier product name. Principles and explanation of the procedure microbiological method. Skirrow campylobacter selective agar sciencedirect.
Bd trypticase soy agar ii with 5% horse blood intended use bd trypticase soy agar ii with 5% horse blood is a nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical specimens and for the detection of hemolytic reactions. It is a highly nutritious medium generally used as a basal medium for preparing blood agar by supplementation with blood. Blood agar plate blood agar plates baps contain mammalian blood usually sheep or horse, typically at a concentration of 510%. Prepare the blood agar base as instructed by the manufacturer. Blood agar base dehydrated thermo fisher scientific. Blood agar is a generalpurpose enriched medium used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. Blood agar bap is a differential growth medium which microbiologists use to distinguish clinically significant bacteria from throat and sputum cultures. Comparison of bacterial count of the different organisms using the statistical differences on the different blood agar with respect to human blood. Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony. Blood agar media blood agar is an enriched media, which capable of growing a range of microorganisms of clinical significance. It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Sheep blood agar base showed considerable improvement and the expected beta haemolytic reactions with s. Basic bacteriology and culture helicobacter pylori ncbi bookshelf.
The characteristic double zone of clear betahemolysis around a colony is clearly seen arrow. Oct 16, 2017 blood agar media blood agar is an enriched media, which capable of growing a range of microorganisms of clinical significance. Sheep blood has been added, in various concentrations, to facilitate the growth of some organisms, and for the observation of hemolytic reactions. Heating of blood agar converts it into chocolate agar heated blood turns a chocolate color and supports the growth of these bacteria. It is useful for cultivating fastidious organisms and for determining. Add 5% sterile defibrinated sheep, rabbit, guinea pig, or horse blood 5 ml blood to 100 ml agar to fullstrength tsa agar base 20 g in 500 ml distilled water. It can also be used as general purpose medium without the addition of blood. Blood agar composition, preparation, uses vs chocolate. The history of blood agar, as we know it today, is uncertain. Antimicrobial susceptibility on solid media basicmedical key.
Recommended for use as general purpose growth media for the isolation, cultivation, and differentiation of a wide variety of microorganisms. Blood agar ba are enriched medium used to culture those bacteria or microbes that do not grow easily. Columbia blood agar base w 1 % agar is used as a base for preparing media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Blood agar plates bap blood agar is actually a couple of related media, all of which are rich formulations containing peptones, yeast extracts, liver or heart extracts depending on the medium, and blood.
Composition ingredients gms litre peptic digest of animal tissue 10. Examples includes crystal violet blood agar to select streptococcus pyogens from throat swabs, and kanamycin or neomycin blood agar to select anaerobes from pus. However, primary isolation is still difficult from clinical specimens. The typical colonies are flat, glossy, and effuse with a tendency to form a spreading film if the agar is moist. Top american libraries canadian libraries universal library community texts project gutenberg biodiversity heritage library childrens library.
Blood agar plates bap are made by adding five to ten percent sheep or horse blood to the nutrient medium. I am just wondering is there another agar plate i could use instead or if. Description oxoid blood agar base is a nonselective general purpose medium widely employed for the growth of pathogenic and nonpathogenic bacteria. A bbl i plate dish offers two highquality bbl media.
Recommended as a base to which blood may be added for use in the isolation and cultivation of fastidious pathogenic microorganisms like neisseria, streptococci etc. The results after incubation of a blood agar plate exposed to a laboratory environment for approximately 2 hrs. Pp1084 columbia agar with 5% defibrinated horse blood. Human blood agar huba is widely used in developing countries for the isolation of bacteria from clinical specimens. Some text book authors refer to alpha as partial hemolysis, which may be. Blood agar tryptic soy agar with 5% sheep blood general. Distilled water since blood agar is made from nutrient agar, above is the composition of nutrient agar 5% sheep blood. Add above components 40 gm, except sheep blood, to distilleddeionized water and bring volume to 950.
It can also be used for primary isolation of haemophilus species, where horse blood is used for enrichment. Grow a wide variety of fastidious organisms on thermo scientific blood agar tsa with sheep blood, a general purpose nonselective medium with the addition of sheep blood in the formulation to promote the presence and visualization of haemolytic reactions so it can be used as a diagnostic tool. True, some bacteria are unable to grow unless blood is used to enrich the agar horse blood agar hba grows a greater variety of organisms than nutrient agar horse blood agar. Skirrow campylobacter selective agar is composed of proteose peptone, liver digest, yeast extract, sodium chloride, vancomycin, polymyxin b, trimethoprim, agar, distilled or deionized water, and lysed horse blood. The chapter also describes the preparation of skirrow campylobacter selective agar. Horse blood agar contains pancreatic digest of casein and soy peptone which provide essential carbon and nitrogen elements to support cell growth. Pp0972 muellerhinton agar with 5% defibrinated horse blood. It is typically tryptic soy agar or columbia agar base with 5% sheep blood. Boiled blood agar chocolate agar is prepared by heating after the blood has been added to the blood agar base. Columbia agar with 5% horse blood is a highly nutritious general purpose medium for the isolation and. Chocolate agar supplemented with 10% sterile defibrinated blood is suited for isolating haemophilus and neisseria species. Columbia agar base supplemented with sheep, rabbit or horse blood derives its. Bd columbia agar with 5% sheep blood intended use bd columbia agar with 5% sheep blood is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from clinical specimens.
However, all horse blood is not necessarily the same, but then neither is all sheep blood. Better results are obtained by spreading half of the horse blood agar plate with 2 drops of 10% saponin. Chocolate agar is used for the isolation of fastidious organisms, such as haemophilus influenzae, when incubated at 3537c in a 5% co2 atmosphere. Bordetella pertussis growth on charcoal horse blood agar. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. Concentrations found in certain media such as peptone water and nutrient agar will virtually eliminate sulfonamide activity 26. Suspend 28 g of nutrient agar powder in 1 litre of distilled water. Blood agar is almost identical to nutrient agar except that it contains five to ten percent sheep, rabbit, or horse blood. Blood agar plates baps contain mammalian blood usually sheep or horse, typically at a concentration of 510%. Fastidious anaerobe agar with 7% horse blood fastidious anaerobe agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The medium needs to be heated to inactivate the inhibitor and.
As a bacterial culture medium, citrated sheep blood agar is a. The composition of chocolate agar and the blood agar is the same and the only difference is while preparing chocolate agar, the red blood. It is an enriched, nonselective differential medium that supports growth of a variety of bacteria and can detect hemolytic activity of the microorganism. Blood agar consists of many ingredients but the primary ingredient is the blood, which came from a rabbit or a sheep. Blood agar composition, principle, preparation and uses. Blood agar is an enriched, bacterial growth medium. Clostridium perfringens lab humor electron microscope lab tech medical laboratory molecular biology medical technology science ideas fungi. Sheep and human blood are not suitable for this purpose, as they contain enzymes that inactivate nad.
Phillips 2, described an improved medium for sporulation of clostridium perfringens based on blood agar base no. Effect of blood agar from different animal blood on growth. There seem to be greater differences between horse and sheep blood agar. Blood agar composition, preparation, uses and pictures. Isolates human blood agar sheep blood agar cow blood agar goat blood agar rabbit blood agar laked horse blood agar chicken blood agar klebsiella pneumoniae 1. Beef extract, peptone, and nicotinic acid provide essential nutrients for the growth of bordetella spp. The composition of chocolate agar and the blood agar is the same and the only difference is while preparing chocolate agar, the red blood cells are lysed. Taken from the wistreich collection, appearing exclusively on microbeworld. Blood agar consists of a base containing a protein source e. Blood agar contains general nutrients and 5% sheep blood. Tryptones, soybean protein digest, sodium chloride nacl, agar and 5% sheep blood. Sheep blood agar plate intended use recommended for cultivation of fastidious organisms and studying haemolytic reactions. Bd columbia agar with 5% horse blood becton dickinson.
Blood agar composition, principle, preparation and uses online. Heat with frequent agitation and boil for 1 min to completely dissolve. The red blood cells remain intact in the agar and make the plates a blood red color. Anaerobic culture of clostridium perfringens on blood agar. Blood agar may be prepared by adding 5%vv sterile defibrinated blood to. Blood agar is universal bacterial growth medium contains mammalian blood usually sheep or horse, typically at a concentration of 510%. Hardy diagnostics horse blood agar is an enriched allpurpose growth medium recommended for the cultivation of nonfastidious and fastidious microorganisms such as haemophilus spp. Tryptic soy agar is the basal medium for the blood agar products. Growth occurs on a cap because nad is released from the blood during the heating process of chocolate agar preparation the. The causative agent was not recognized previously, since it does not grow on conventional agar such as sheep blood agar. It can be prepared with heatlysed horse blood, which is a good source of both hemin and nad, although sheep blood can also be used. Blood agar plate, 5% sheep blood in tryptic soy agar tsa base, 15x100mm plate, order by the package of 10, by hardy diagnostics. Preston agar base m939 preston agar base with added supplement is recommended for selective isolation of thermotolerant campylobacter species. This study compared citrated sheep blood agar csba and huba with defibrinated horse blood agar and defibrinated sheep blood agar dsba.
Summary tryptic soy agar tsa is a popular allpurpose growth medium used to support a wide variety of microorganisms. Freshly drawn blood from healthy animals can destroy small. Bordetella pertussis in particular, requires the addition of charcoal and horse blood whole, 10% to neutralize the growthinhibiting effects. Heat this mixture while stirring to fully dissolve all. Bordetella pertussis growth on charcoal horse blood agar with. Blood agar can be made selective for certain pathogens by the addition of antibiotics, chemicals or dyes. Oct 26, 2018 blood agar can be made selective for certain pathogens by the addition of antibiotics, chemicals or dyes. Additional supplementation of the mueller hinton medium using 5% horse blood and 20mgl of nicotinamide adenine dinucleotide nad makes it suitable for use with the more fastidious organisms such as streptococcus pneumoniae and haemophilus influenzae. It is primarily used to grow fastidious microorganisms like streptococci. Selective media contain ingredients that inhibit the growth of some organisms but allow others to grow.
The blood is usually sheeps red blood cells rbc, though horse and other species may be used. Defibrinated sheep, horse, pig, or goat blood agar is recommended for the isolation of s. The medium becomes chocolate brown in colour, then pour plates. It is enriched, differential media used to isolate. For blood agar, cool the base to 50c and add 7% of defibrinated horse blood sr0050. Grows chocolate agar but not nutrient agar or horse blood agar.
But in text books of microbiology goat blood is not advised for this purpose. To read the hemolytic reaction on a blood agar plate, the plate must be held up to a light. Blood agar base is a highly nutritious medium generally used as a basal medium for preparing blood agar by the addition of blood. Cap has a reduced concentration of agar, which increases the moisture content of the medium. Horse blood agar for the cultivation of haemophilus spp.
Fastidious organisms, such as streptococci, do not grow well on. The composition of a particular culture medium formulation. Most clinical microbiology laboratories still rely upon cu. An agar plate is a petri dish that contains agar as a solid growth medium plus nutrients, used to. The blood is completely digestedall that is left is the agar. Full text of the use of blood agar for the study of. Should horse or sheep blood agar be used to identify.
Agar prepared with human blood is not recommended, partly because of the safety risk to laboratory personnel, but mainly because it is said to result in poor bacterial isolation rates, although there are few published data to support this 2. As a bacterial culture medium, citrated sheep blood agar. Horse blood agar is a type of bloodenriched microbiological culture media. In our experience it is important to educate staff on the clinical relevance and importance of this rather. The haemolytic reactions of horse and sheep blood are not identical and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa. Mix with gentle rotation and pour into petri dishes or other containers. Full text of the use of blood agar for the study of streptococci. Susceptibility testing agar used in any of the current published methods has minimal concentrations of paba. Tryptose serves as nitrogen source while sodium chloride maintains osmotic equilibrium. Hemolytic properties described in diagnostic textbooks usually refer to sheep blood. While evolving molecular diagnostic methods are being heralded for the role they will play in improving our ability to cultivate and identify bacteria, fungi, and viruses, the reality is that those new methods are still beyond the technical and financial reach of most clinical laboratories.
1122 7 575 58 1557 979 916 29 173 1559 1619 846 16 1284 988 157 575 462 1464 541 77 1127 1443 795 1146 1326 1213 1478 809 1296 670